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Temperature project

REL1206 and REL1207 are the Ara- and Ara+ ancestors, respectively, for the Bennett & Lenski temperature project. REL1206 is a clone sampled from population A-1 at generation 2,000 of the main long-term experiment. REL1206 and REL1207 are isogenic, with the exception of a spontaneous mutation to Ara+ in REL1207. Six populations were propagated under each of five thermal regimes. In each group, three were founded with each ancestor. The following strains are all from generation 2,000 of the temperature evolution. For example, REL2052 refers to the isolate from replicate A-1 in the switch regime that alternated between 32°C and 42°C each day.

Population

REL#

Population

REL#

32/-1 2037 Sw/-1 2052
32/+1 2038 Sw/+1 2053
32/-2 2039 Sw/-2 2054
32/+2 2040 Sw/+2 2055
32/-3 2041 Sw/-3 2056
32/+3 2042 Sw/+3 2057
37/-1 2143 20/-1 7000
37/+1 2043 20/+1 7001
37/-2 2044 20/-2 7002
37/+2 2045 20/+2 7003
37/-3 2046 20/-3 7004
37/+3 2144 20/+3 7005
42/-1 2047
42/+1 2142
42/-2 2048
42/+2 2049
42/-3 2050
42/+3 2051

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